IMMUNOHISTOCHEMICAL RESEARCH OF FOLLICULAR THYROID TUMORS 

Kalmin Oleg Vital'evich, Doctor of medical sciences, professor, head of sub-department of human anatomy, Medical Institute, Penza State University (40 Krasnaya street, Penza, Russia), ovkalmin@gmail.com
Chairkin Ivan Nikolaevich, Doctor of medical sciences, professor,  sub-department of human anatomy, I. M. Sechenov First Moscow State Medical University (8 Trubetskaya street, Moscow, Russia), chairkin@rambler.ru
Kalmin Oleg Olegovich,  Assistant, sub-department of human anatomy, Medical Institute, Penza State University (40 Krasnaya street, Penza, Russia), kalmin.o.o@gmail.com

 616.441-006.55+.6

 10.21685/2072-3032-2017-1-3

Background. The aim of the research was to identify the immunohistochemical features of thyroid structure in different types of follicular tumors.
Matherials and methods. 6 cases of follicular cancer and follicular adenoma were observed. A commercial kit EnVision produced by Dako company was used as universal secondary antibodies and a visualization system. After that, nuclei were counterstained by hematoxylin. The evaluation of results of immunohistochemical reactions was performed taking into account the localization, extent and intensity of tumor cells staining. In order to study morphological features of follicular tumors of different degree of malignancy on sections stained with hematoxylin-eosin, the authors conducted a morphometric study with definition of quantitative parameters.
Results and conclusions. The study showed an increased expression of all the investigated antigens in cells in follicular carcinoma, which distinguished it from follicular  adenoma. But thus, as long as many antigens can also be identified in follicular adenoma, the insufficient specificity does not allow using only one insulated marker due to an increased risk of wrong diagnosis of follicular tumor. Differences in the sizes of tumor cells and their nuclei can also help in differential diagnosis of follicular adenoma and follicular cancer. However, only the integrated use of immunohistochemical and morphometric methods allows to greatly increase the accuracy of the differential diagnosis of thyroid tumors with a similar morphological structure.

thyroid gland, follicular adenoma, follicular cancer, immunohistochemistry, galectin-3, cytokeratin-19, НВМЕ-1.

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